Free research tools
Free Semaglutide Reconstitution Calculator
Free·No sign-up·Instant
Reconstitute Semaglutide accurately. The calculator below is pre-loaded with a typical Semaglutide starting point — adjust vial strength, bacteriostatic water and your target dose to read the exact volume and U-100/U-40 syringe units to draw. For research use only.
Draw to
mL
units
- Concentration
- /mL
- Dose
- mg ( mcg) IU
- Add BAC water
- mL
- Doses per vial
- Lasts ~
- days
/ on a syringe
Helica Labs · Research Use Only
Semaglutide Reconstitution & Dosing Chart
Reference values for a 5 mg Semaglutide vial. Doses shown are research reference points for the reconstitution math, not medical guidance. Use the calculator above for other vial strengths or target doses.
Bacteriostatic water → concentration
| BAC water | Concentration | 1 mg = |
|---|---|---|
| 1 mL | 5 mg/mL | 20 units (U-100) |
| 2 mL | 2.5 mg/mL | 40 units (U-100) |
| 3 mL | 1.67 mg/mL | 60 units (U-100) |
Dose → draw (5 mg in 2 mL = 2.5 mg/mL)
| Dose | Draw | U-100 units | U-40 units |
|---|---|---|---|
| 0.25 mg | 0.1 mL | 10 | 4 |
| 0.5 mg | 0.2 mL | 20 | 8 |
| 1 mg | 0.4 mL | 40 | 16 |
| 1.7 mg | 0.68 mL | 68 | 27.2 |
| 2.4 mg | 0.96 mL | 96 | 38.4 |
For research use only. Not medical advice. Verify every value against current literature and your syringe graduation before use. A draw over 100 U-100 units exceeds a 1 mL insulin syringe — split the dose or use a larger barrel.
How to mix Semaglutide with bacteriostatic water
- Swab the rubber stopper of both the Semaglutide vial and your bacteriostatic water vial with an alcohol wipe and let them dry.
- Draw your chosen amount of bacteriostatic water into the syringe (use the calculator above to pick a volume that lands your dose on round units).
- Inject the water slowly down the inside glass wall of the Semaglutide vial — aim the stream at the glass, never directly onto the powder.
- Gently swirl or roll the vial until the solution is completely clear. Do not shake.
- Store the reconstituted Semaglutide refrigerated, then use the calculator and chart above to read the exact mL and syringe units to draw.
Frequently asked questions
- What is the difference between a U-100 and U-40 syringe?
- A U-100 syringe holds 100 units per mL (1 unit = 0.01 mL); a U-40 holds 40 units per mL (1 unit = 0.025 mL). The same volume reads a different number of units on each, so always match the calculator to your syringe.
- Is there a U-50 syringe?
- No. A "50-unit syringe" is simply a 0.5 mL U-100 syringe — it holds 50 units. There is no U-50 insulin concentration.
- How much bacteriostatic water should I add?
- Any amount works mathematically; more water means a larger, easier-to-measure draw but a more dilute solution. Use the reverse-solve mode to find the BAC water that lands your dose on a round number of units.
- How much bacteriostatic water for a 5 mg Semaglutide vial?
- Adding 2 mL of bacteriostatic water to a 5 mg vial gives 2.5 mg/mL, so 1 mg reads 40 units on a U-100 syringe. More water makes a larger, easier-to-measure draw but a more dilute solution — use the calculator above to land your target dose on round units. Research use only.
- How much bacteriostatic water for a 10 mg Semaglutide vial?
- Adding 2 mL of bacteriostatic water to a 10 mg vial gives 5 mg/mL, so 1 mg reads 20 units on a U-100 syringe. More water makes a larger, easier-to-measure draw but a more dilute solution — use the calculator above to land your target dose on round units. Research use only.
For research use only. Verify all calculations against current literature and use sterile technique.